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Cell and Tissue Culture Flasks

Cell and tissue culture flasks are the most suitable culture vessels for long-term and large-scale laboratory cell culture while preventing contamination. The surface untreated flasks are suitable for suspension cells culture, while those with a TC-treated surface are suitable for common adherent cell lines due to the excellent hydrophilicity of the polystyrene surface. The CellATTACH® superhydrophilic treated surface represents a technical advancement that improves the adhesion and growth of difficult-to-nourish cells, primary cells and transfection cell lines under low serum/serum-free conditions.


Specification: T12.5 T25 T75 T150 T175 T182 T225 T300

Type of Cap:   Plug Seal    Vent

Surface: Non-treated    TC treated    CellATTACH® treated


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Product descriptionDownload


Flask Body: Polystyrene (PS)
Bottle Cap: High-density Polyethylene (HDPE)
Filter Membrane: Polytetrafluoroethylene (PTFE), 
conforming to USP Class VI standards



* Various treated surfaces are suitable for different culture needs
* The hydrophobic vent cap ensures continuous ventilation, enabled by turning the cap 1/4 of its full range
* The tilted bottleneck facilitates pipets and cell scrapers for easy access
* Low profile design supports effective use of the internal space of the incubator when stacked
* The frosted area near the bottleneck can be written on
* Volume graduations molded on both sides
* 100% tested for production line leakage
* Lot No. on the bottom of each flask and package bag for quality traceability
* Sterilized by irradiation, SAL 10-6
* DNase/RNase free, non-pyrogenic and non-cytotoxic


Ordering information


Cell and Tissue Culture Flasks , Non-treated

Cat. No. Volume(mL) Culture Surface Area(cm²) Type of Cap Recommended Working Volume(mL) Maximum Working Volume(mL) Length(mm) Width(mm) Height(mm) Botte Neck Diameter(mm) Sterile Qty. Per Bag Qty. Per Case
TCF001025 25 12.5 Plug Seal / 8 8 73.71 40.38 22.79 Y 10 200
TCF002025 25 12.5 Vent 8 8 73.71 40.38 22.79 Y 10 200
TCF001050 50 25 Plug Seal 17.5 17.5 92.88 49.5 29.08 Y 10 200
TCF002050 50 25 Vent 17.5 17.5 92.88 49.5 29.08 Y 10 200
TCF001250 250 75 Plug Seal 60 60 152.52 81.54 35.21 Y 5 100
TCF002250 250 75 Vent 60 60 152.52 81.54 35.21 Y 5 100
TCF001150  375 150 (U-shaped) Plug Seal 140 140 199.7 111.3 111.3 Y 5 50
TCF002150 375 150 (U-shaped) Vent 140 140 199.7 111.3 111.3 Y 5 50
TCF001175 600 175 Plug Seal 250 250 199.9 122.7 49.2 Y 5 50
TCF002175 600 175 Vent 250 250 199.9 122.7 49.2 Y 5 50
TCF001600 600 182 Plug Seal 125 125 219.28 115.74 38.3 Y 5 40
TCF002600 600 182 Vent 125 125 219.28 115.74 38.3 Y 5 40
TCF101600 600 182 (Extended) Plug Seal 200 200 219.28 115.74 49.5 Y 5 40
TCF102600 600 182 (Extended) Vent 200 200 219.28 115.74 49.5 Y 5 40
TCF001225 850 225 Plug Seal 400 400 221.9 137.2 49.5 Y 5 25
TCF002225 850 225 Vent 400 400 221.9 137.2 49.5 Y 5 25
TCF001850 850 300 Plug Seal 200 200 269.15 166 47 Y 3 18
TCF002850 850 300 Vent 200 200 269.15 166 47 Y 3 18

Cell and Tissue Culture Flasks, TC treated

Cat. No. Volume(mL) Culture Surface Area(cm²) Type of Cap Recommended Working Volume(mL) Maximum Working Volume(mL) Length(mm) Width(mm) Height(mm) Botte Neck Diameter(mm) Sterile Qty. Per Bag Qty. Per Case
TCF011025 25 12.5 Plug Seal 2.5-3.75 8 73.71 40.38 22.79 14.15 Y 10 200
TCF012025 25 12.5 Vent 2.5-3.75 8 73.71 40.38 22.79 14.15 Y 10 200
TCF011050 50 25 Plug Seal 5-7.5 17.5 92.88 49.5 29.08 18.2 Y 10 200
TCF012050 50 25 Vent 5-7.5 17.5 92.88 49.5 29.08 18.2 Y 10 200
TCF011250 250 75 Plug Seal 15-22.5 60 152.52 81.54 35.21 25 Y 5 100
TCF012250 250 75 Vent 15-22.5 60 152.52 81.54 35.21 25 Y 5 100
TCF011150 375 150 (U-shaped) Plug Seal 30-45 140 199.7 111.3 111.3 25.6 Y 5 50
TCF022150  375 150 (U-shaped) Vent 30-45 140 199.7 111.3 111.3 25.6 Y 5 50
TCF011175 600 175 Plug Seal 35-52.5 250 199.9 122.7 49.2 25.7 Y 5 50
TCF012175 600 175 Vent 35-52.5 250 199.9 122.7 49.2 25.7 Y 5 50
TCF011600 600 182 Plug Seal 36.4-54.6 125 219.28 115.74 38.3 29.5 Y 5 40
TCF012600 600 182 Vent 36.4-54.6 125 219.28 115.74 38.3 29.5 Y 5 40
TCF111600  600 182 (Extended) Plug Seal 36.4-54.6 200 219.28 115.74 49.5 29.5 Y 5 40
TCF112600 600 182 (Extended) Vent 36.4-54.6 200 219.28 115.74 49.5 29.5 Y 5 40
TCF011225 850 225 Plug Seal 60-90 400 221.9 137.2 49.5 25.65 Y 5 25
TCF012225 850 225 Vent 60-90 400 221.9 137.2 49.5 25.65 Y 5 25
TCF011850 850 300 Plug Seal 60-90 200 269.15 166 47 29.5 Y 3 18
TCF012850 850 300 Vent 60-90 200 269.15 166 47 29.5 Y 3 18


Cell and Tissue Culture Flasks, CellATTACH® treated

Cat. No. Volume(mL) Culture Surface Area(cm²) Type of Surface Maximum Working Volume(mL) Recommended Working Volume(mL) Type of Cap Sterile Qty. Per Bag Qty. Per Case
CAF011025 25 12.5 CellATTACH® Treated 8 2.5 - 3.75 Plug Seal Y 10 200
CAF012025 25 12.5 8 2.5 - 3.75 Vent Y 10 200
CAF011050 50 25 17.5 5 - 7.5 Plug Seal Y 10 200
CAF012050 50 25 17.5 5 - 7.5 Vent Y 10 200
CAF011250 250 75 60 15 - 22.5 Plug Seal Y 5 100
CAF012250 250 75 60 15 - 22.5 Vent Y 5 100
CAF011600 600 182 125 36.4 - 54.6 Plug Seal Y 5 40
CAF012600 600 182 125 36.4 - 54.6 Vent Y 5 40
CAF111600 600.0 182 Extended 200 36.4 - 54.6 Plug Seal Y 5 40
CAF112600 600.0 182Extended 200 36.4 - 54.6 Vent Y 5 40
CAF011850 850 300 200 60 - 90 Plug Seal Y 3 18
CAF012850 850 300 200 60 - 90 Vent Y 3 18




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