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ELISA Plates

As an important tool for ELISA (enzyme linked immunosorbent assay) experiments, ELISA plates are made of PS. Antigens, antibodies and biomolecules are binding on the bottom surface of the plate by means of hydrophobic and ionic bonds. The plates are mainly used in disease and food safety diagnosis.

The ELISA plates from Jet Biofil are made with internationally advanced surface treatment technologies and manufacturing processes for high polymer materials, and show stable protein-binding properties. They can be used as safe, reliable and effective carriers during ELISA experiments, and in conjunction with immune and genetically modified products, as well as for clinical diagnosis.



Specification: 96-well non-removable plate, 96-well/48-well removable plate (fitted with 8-well strip or 12-well strip)
Binding Force: High binding force   Moderate binding force
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* Polystyrene (PS) and High Impact Polystyrene (HIPS)

* Conforming to USP Class VI standard



* Unique surface treatment process for higher protein adsorption properties
* 2 binding forces available: High binding force (300~400ng/cm²), and moderate binding force (200~300ng/cm²)
* 8-well and 12-well strips are provided to match the ELISA plates and for better cost-effectiveness
* Designed with a flat bottom and divided into removable and non-removable structures, to satisfy different experimental applications
* Even well diameter and thickness, ensuring high experimental accuracy and repeatability
* Transparent plate, with a CV value <5%, higher measurement flexibility, widely used in colorimetric determination
* Clearly marked with letters and numbers, to distinguish the samples in different wells
* Dimensions conform to SBS international standards, and are stable for most brands of ELISA equipment
* Sterilized by irradiation, SAL 10-6
* DNase/RNase-free, non-pyrogenic


High binding force ELISA plate
The plate undergoes surface treatment to increase protein binding force, up to 300~400ng/cm² (IgG); molecular weight of binding protein: >10kD. This type of ELISA plate can improve sensitivity and reduce the coing protein concentration and usage. If they were not present, the non-ionic detergent to fail to blocking the binding protein, nonspecific reactions could occur, meaning the protein would need to be used as a blocking agent.


Moderate binding force ELISA plate
The ELISA plate binds with proteins through hydrophobic bonds on the surface, and is suitable for use as a solid phase carrier to macromolecule proteins with a molecular weight >20kD, protein binding capability is 200-300ng /cm²(IgG). As the ELISA plate only displays macromolecular binding, it is also suitable as a solid phase carrier for unpurified antibodies or antigens. Proteins or non-ionic detergents can be used as blocking liquid on these plates.



Type of ELISA Plate

Transmittance Variation (CV)

Binding Action

Sample Characteristics

Recommended Sealing Agent

High binding force plate

300 ~ 400ng/cm²

IgG


<5.00%

Hydrophobic bond

Middle/macromolecular protein with positive charge >10kD

PBS containing 0.3% Tween 20, combination of 0.05% Tween 20 and 1%BSA

Moderate binding force plate

200 ~ 300ng/cm²

IgG


Hydrophobic bond/ionic bond

Macromolecular protein >20KD

Detergent Tween 20 is used in combination with protein, BSA, skim milk and serum

Ordering information

Cat. No.

Well Qty.

Binding Capacity

Description

Qty.Per (Bag/Box)/Case

FEP100096

96 well

High Binding

Fixed Flat Bottom

10/200

FEP111096

96 well

High Binding

Detachable, 8x12

10/200

FEP101896

96 well

High Binding

Detachable, 8x12

10/200

FEP101296

96 well

High Binding

Detachable, 12x8

10/200

FEP100012

12 well

High Binding

Strip 12x8

40/1600

FEP100008

8 well

High Binding

Strip 8x12

60/2400

FEP200096

96 well

Medium Binding

Fixed Flat Bottom

10/200

FEP201896

96 well

Medium Binding

Detachable, 8x12

10/200

FEP201296

96 well

Medium Binding

Detachable, 12x8

10/200

FEP200012

12 well

Medium Binding

Strip 12x8

40/1600

FEP200008

8 well

Medium Binding

Strip 8x12

60/2400

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