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CellDETACH™ Temperature-Responsive Cell Culture Surface

Using trypsin digestion or cell scrapers to separate adherent cells can affect the expression of cell surface proteins, damage cells and reduce cell viability.
The CellDETACHTM products have a thermosensitive surface, which is coated with a unique nanopolymer. When the temperature decreases from 37°C to 4°C, the thermosensitive surface gradually changes from slightly hydrophobic to hydrophilic, allowing for the harvest of adherent cells without trypsin. By using this gentle collection method, the cells are safe from injuries caused by trypsin or scrapers, thus preserving high viability and the integrity of surface receptors and cell antigens. This operation enables cells to be harvested without damage for subculturing.



Products: 
CellDETACH™ thermosensitive cell culture dishes 100mm

CellDETACH™ thermosensitive cell culture flasks 600mL


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Product descriptionDownload

The CellDETACH™ thermosensitive cell culture surface is specially designed by our R&D team for cell passage and cell transplantation, and has been granted a national invention patent (Patent Number: ZL201510780506.3). The goal is to help researchers harvest cell sheets, set up 3D tissue models formed by normal cell binding and extracellular matrix linkage, simplify cell culture and tissue engineering techniques, and minimize experimental manipulation time.


* National invention patent (Patent Number: ZL201510780506.3)
* Induces cell shedding simply by lowering the temperature - straightforward, fast, and easy to operate
* No trypsin: preserves cell surface proteins and markers intact
* No cell scraping: avoids mechanical damage to cells and ensures high cell viability
* Optimized cell culture and tissue engineering techniques


Scope of Application
The thermosensitive cell culture surface is suitable for the in vitro culture of most adherent cells, including stem cells, neural cells, macrophages, and cancer cells. It is ideal for harmless cell harvesting, and can be widely used in expanded cell culture, cell therapy, 3D tissue modeling, extracellular matrix research and other fields.
Instructions for Use
In Vitro Cell Culture
1. When the temperature is above 32℃, the thermosensitive coating on the surface of the CellDETACH™ products is in a curled polymer state, showing slight hydrophobicity. This facilitates cell adherence and growth.
2. When the temperature goes below 32°C, the thermosensitive coating on the surface is in a stretched polymer state, which will bind water molecules and expand, showing hydrophilicity. This facilitates the shedding of adherent cells. The shedding efficiency will be at its best when the temperature drops below 4 °C.
3.When the temperature of the thermosensitive cell culture surface drops below 32°C, excessive disturbance may cause cells to fall off, so please do not spend too much time taking pictures and observing while performing the cell culture.
Cell Harvest
* The best harvest is achieved when the confluence of cells is higher than 80%.
* When harvesting cells, either the environmental temperature should be reduced to 4 ℃ , or the thermosensitive products should be placed in a sterile incubator at  4 ℃, or the culture can be replaced with a 4 °C culture medium.
* When the temperature of the thermosensitive Cell Culture Surface drops to 4 ℃, keep it for 20 ~ 30 minutes, then aspirate the culture medium above the thermosensitive Cell Culture Surface with a straw (cell culture dish), pipette or electric pipette (600ml Cell Culture Flask), and blow over the cells attached to the culture surface to make them fall off. During the blowing process, the cell sheet can be observed falling off the thermosensitive surface.
* The thermosensitive shedding abilities of cells depends on their adhesion performances, and some cells with strong adherence may have difficulty falling off, requiring multiple blowing attempts. (for example, those digested at 37 ℃ for more than 3 minutes with 0.25% trypsin digestion are considered to be cells with strong adherence ability).
Storage and Transportation
* This product should not be exposed to direct sunlight or excessive heat for a long time, but can be stored and transported at room temperature.









Ordering information

Cell Culture Dishes

Cat. No. Diameter(mm) Sterile Appro.Cell Growth Area (cm²) Packaging
Qty. Per
Pack(Blister)
Qty. Per
Case
CDD022100 100 Y 60.8 Blister 1 24
CDD023100 100 Y 60.8 Pack 5 100


Cell Culture Flasks

Cat.No.
Volume
(mL)
Cell Culture
Surface
Area (cm2
)
Recommended
Working
Volume(mL)
Maximum
Working Volume (mL)
Cap
Style
Dimensions(mm) Sterile
Qty. Per
Bag
Qty. Per
Case
L W H B.N.D*
CDF024600 600 182(Extended) 36.4-54.6 200 Vent 219.28 115.74 49.5 29.5 Y 1 20
CDF023600 600 182(Extended) 36.4-54.6 200 Vent 219.28 115.74 49.5 29.5 Y 5 40
CDF014600 600 182(Extended) 36.4-54.6 200 Plug Seal 219.28 115.74 49.5 29.5 Y 1 20
CDF013600 600 182(Extended) 36.4-54.6 200 Plug Seal 219.28 115.74 49.5 29.5 Y 5 40



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